A study on Antimicrobial Resistance of Helicobacter Pylori Isolates Recovered from Dyspeptic Patients by Epsilometer Test

Authors

  • Alireza Massiha Department of Biotechnology, Lahijan branch, Islamic Azad University of Iran
  • Mohammad Reza Majid Khoshkholgh Pahlaviani Department of Biotechnology, Lahijan branch, Islamic Azad University of Iran
Abstract:

The main purpose of the study is to determine resistance pattern of bacterial isolates to selected antibiotics among 88 dyspeptic patients in Iran. Frequency of infection among these patients have been evaluated among dyspeptic patients old (45 boys and 43 girls, aged 5-18 years) from 2016 to 2017.Eighty-eight patients with gastroduodenal disorders were included in the study. Urease test modified Giemsa stain, culture isolation and histopathology of H.pylori isolates were performed as per standard guidelines. H.pylori ATCC 43504 was used as reference strain. Susceptibility of HP isolates to amoxicillin, tetracycline, metronidazole, clarithromycin and levofloxacin was determined using EUCAST break points by E-test method. Antral gastritis caused by H pylori infection (15.91%) with or without extension into the duodenum was prominent endoscopic finding. The study revealed modified Giemsa stain positivity of 13.6%, urease test positivity 23.9%, and culture positivity rate of 18.2%. The three tests Culture, Giemsa, and Urease test found to be significant statistically (P

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Journal title

volume 7  issue 2

pages  824- 833

publication date 2017-12-01

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